Western blot analysis of extracts from HeLa cells treated with Calyculin A #9902 (100nM for 20 minutes) and transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® NDRG1 siRNA I #6245 (+) or SignalSilence ® NDRG1 siRNA II (+), using Phospho-NDRG1 (Ser330) Antibody #3506 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Phospho-NDRG1 (Ser330) Antibody confirms silencing of NDRG1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.